中文
英语
人白细胞介素6检测试剂盒
英文名称:Human IL-6 ELISA Kit
应用: Sandwich ELISA,ELISA
反应性:Human
样品类型:血清/血浆/细胞培养上清
灵敏度:2pg/mL
储存条件:2-8℃，6 months
产品简介
| 中文名称 | 人白细胞介素6检测试剂盒 |
| 英文名称 | Human IL-6 ELISA Kit |
| 别名 | B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; Interferon beta-2 |
| 储存条件 | 2-8℃，6 months |
| 检测目标 | IL-6 |
| 样品类型 | 血清/血浆/细胞培养上清 |
| 反应性 | Human |
| 反应类型 | Sandwich-ELISA |
| 定量/定性 | 定量检测 |
| 灵敏度 | 2pg/mL |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| Swiss Prot | P05231 |
| Gene ID | 3569 |
| 试剂盒类型 | 传统ELISA试剂盒 |
| 检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人IL-6单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人IL-6会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人IL-6抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人IL-6发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人IL-6，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人IL-6浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人IL-6的浓度。 |
| 规格 | 48T 96T |
| 应用 | Sandwich ELISA ELISA |
| 疾病系列 | 肥胖研究相关因子 骨质疏松症研究相关因子 冠心病研究相关因子 慢阻肺COPD研究相关因子 骨关节炎相关因子 神经系统疾病相关因子 糖尿病研究相关因子 系统性红斑狼疮相关因子 哮喘相关因子 心血管疾病相关因子 |
| 研究领域 | 神经学 新陈代谢 肿瘤&癌症 免疫学 |
| 单位 | 盒 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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Hua Xu, Jiafu Li, Xin Yu, Huihong Zhang, Hongmei Gao, Bing Wang, Yongqiang Wang
IF：
3.9320
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Annals of Translational Medicine
PMID：
33569452
LncRNA KCNQ1OT1 knockdown inhibits ox-LDL-induced inflammatory response and oxidative stress in THP-1 macrophages through the miR-137/TNFAIP1 axis
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Author：
Can Xu, Lei Chen, Ru-Jing Wang, Jun Meng
IF：
3.9260
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CYTOKINE
PMID：
35598525
LncRNA JHDM1D-AS1 Suppresses MPP + -Induced Neuronal Injury in Parkinson’s Disease via miR-134-5p/PIK3R3 Axis
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Author：
Wang Cuihui, Zhang Hongying, Li Ji
IF：
3.9110
Publish_to：
NEUROTOXICITY RESEARCH
PMID：
34773593
Effect of Zishen pingchan granules combined with pramipexole on serum BDNF, IL-1汕, IL-6, CRP, TNF-汐 levels in depressed patients with Parkinson's disease: Results of a randomized, double-blind, controlled study
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Author：
Houxu Ning, Hao Zhou, Ning Yang, Jingru Ren, Haidong Wang, Weiguo Liu, Yang Zhao
IF：
3.9000
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EXPERIMENTAL GERONTOLOGY
PMID：
37734668
Erythropoietin promotes energy metabolism to improve LPS-induced injury in HK-2 cells via SIRT1/PGC1-α pathway
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Author：
Li Kan, Gao Li, Zhou Sen, Ma Yan-Rong, Xiao Xiao, Jiang Qian, Kang Zhi-Hong, Liu Ming-Long, Liu Tian-Xi
IF：
3.8420
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MOLECULAR AND CELLULAR BIOCHEMISTRY
PMID：
36001204
Dabie bandavirus infection induces macrophagic pyroptosis and this process is attenuated by platelets
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Author：
Sicong Yu, Qinyi Zhang, Lingxuan Su, Ji He, Wen Shi, Hao Yan, Haiyan Mao, Yi Sun, Dongqing Cheng, Xuan Wang, Yanjun Zhang, Lei Fang
IF：
3.8000
Publish_to：
PLoS Neglected Tropical Diseases
PMID：
37486928
Tanshinone IIA-Loaded Micelles Functionalized with Rosmarinic Acid: A Novel Synergistic Anti-Inflammatory Strategy for Treatment of Atherosclerosis
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Author：
Meixuan Liu, Sha Liu, Xiaosu Zhu, Yiying Sun, Linyu Su, Hairong Yu, Deshuai Liu, Ying Li, Yuan Du, Rongxia Liu, Kaoxiang Sun
IF：
3.7840
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JOURNAL OF PHARMACEUTICAL SCIENCES
PMID：
35580692
Clofazimine inhibits innate immunity against Mycobacterium tuberculosis by NF-κB
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Author：
Xinda Li, Xiaoyi Luo, Bin Wang, Lei Fu, Xi Chen, Yu Lu
IF：
3.7000
Publish_to：
mSphere
PMID：
39046230
Circ_0003972 Promotes the Proliferation and Inflammation of Fibroblast-like Synovial Cells in Rheumatoid Arthritis through Regulation of the miR-654-5p/FZD4 Axis
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Author：
Juan Li, Dan Liu, Kunyi Wu, Qun Chen, Jianhong Lei
IF：
3.6570
Publish_to：
IMMUNOLOGICAL INVESTIGATIONS
PMID：
34325604
Circ_0000566 contributes oxygen–glucose deprivation and reoxygenation (OGD/R)-induced human brain microvascular endothelial cell injury via regulating miR-18a-5p/ACVR2B axis
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Author：
Liu Dan, Xiao Haitao, Liu Jinxing, Zhang Yixin, Li Jialiang, Zhang Tingyu, Chen Honglin
IF：
3.6000
Publish_to：
METABOLIC BRAIN DISEASE
PMID：
36781583
Exosomes miR-92a-3p from human exfoliated deciduous teeth inhibits periodontitis progression via the KLF4/PI3K/AKT pathway
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Author：
Tianliang Yu, Na Mi, Yingtao Song, Weili Xie
IF：
3.4000
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JOURNAL OF PERIODONTAL RESEARCH
PMID：
38616305
Structural and biofunctional evaluation of decellularized jellyfish matrices
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Author：
Wen-Bo Wu, Xiao-Fei Jiang, Ming-Quan Chen
IF：
3.3000
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KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
PMID：
37306210
CircLPAR1/miR-212-3p/ZNF217 feedback loop promotes amyloid β-induced neuronal injury in Alzheimer’s Disease
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Author：
Lifang Wu, Qiang Du, Congcong Wu
IF：
3.2520
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BRAIN RESEARCH
PMID：
34403662
Dexmedetomidine activates the PKA/CREB pathway and inhibits proinflammatory factor expression through β2 adrenergic receptors.
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Author：
Baocheng Zhang, Jie Shen
IF：
3.2000
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Immunity Inflammation and Disease
PMID：
38411331
Circular RNA Circ-BANP Regulates Oxidized Low-density Lipoprotein-induced Endothelial Cell Injury Through Targeting the miR-370/Thioredoxin-interacting Protein Axis
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Author：
Chen Guang, Li Yongjun, Zhang Ailing, Gao Lijun
IF：
3.1050
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JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
PMID：
33298736
miR-130a-3p enhances autophagy through the YY1/PI3K/AKT/mTOR signaling pathway to regulate macrophage polarization and alleviate diabetic retinopathy
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Xiaoting Xi, Xuewei Wang, Jia Ma, Qianbo Chen, Yuxin Zhang, Yaxian Song, Yan Li
IF：
3.1000
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Journal of Diabetes Investigation
PMID：
39668747
A Novel MIR503HG/miR-497-5p/CCL19 Axis Regulates High Glucose-Induced Cell Apoptosis, Inflammation, and Fibrosis in Human HK-2 Cells
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Author：
Zhang Danping, Chen Xiaoxiao, Zheng Dan
IF：
3.0940
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
PMID：
35013925
Modified TPP-MoS2 QD Blend as a Bio-Functional Model for Normalizing Microglial Dysfunction in Alzheimer＊s Disease
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Ohoud A. Alomari, Safaa Qusti, Maha Balgoon, Fadwa Aljoud, Khalid A. Alamry, Mahmoud A. Hussein
IF：
3.0000
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Neurology International
PMID：
37606394
AKR1B10 expression in benign prostatic hyperplasia and its related mechanism
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Author：
Wenjing Xu, Ya Gao, Jiaqi Zhang, Rong Zhang, Qihua Chen
IF：
2.9670
Publish_to：
Oncology Letters
PMID：
34434282
Fluorofenidone attenuates paraquat‑induced pulmonary fibrosis by regulating the PI3K/Akt/mTOR signaling pathway and autophagy
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Author：
Feiya Jiang, Sha Li, Yu Jiang, Zhuo Chen, Tongtong Wang, Wen Liu
IF：
2.9520
Publish_to：
Molecular Medicine Reports
PMID：
33786626
HSF1 Increases EOGT-Mediated Glycosylation of Notch1 to Promote IL-1β-Induced Inflammatory Injury of Chondrocytes
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Author：
Yuanchi Huang, Wenjie Pan, Huanli Bao, Xiangxiang Sun, Chao Xu, Jianbing Ma
IF：
2.8000
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Cartilage
PMID：
38366389
LncRNA UCA1 negatively regulates NF-kB activity in psoriatic keratinocytes through the miR125a-A20 axis
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Author：
Xiao-Lei Ma, Guang-Dong Wen, Cong Yu, Zheng Zhao, Na Gao, Zheng-Yi Liu
IF：
2.7440
Publish_to：
KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
PMID：
33554442
Triptolide improves Alzheimer’s disease by regulating the NF signaling pathway through the lncRNA NEAT1/microRNA 361 3p/TRAF2 axis
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Author：
Li Zhou, Xuming Huang, Haiyan Li, Jihui Wang, Zhengqi Lu
IF：
2.7000
Publish_to：
Experimental and Therapeutic Medicine
PMID：
37614428
Inhibitory Effect of Paeonol on Apoptosis, Oxidative Stress, and Inflammatory Response in Human Umbilical Vein Endothelial Cells Induced by High Glucose and Palmitic Acid Induced Through Regulating SIRT1/FOXO3a/NF-κB Pathway
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Author：
Hanqing Tang, Keming Li, Shitian Zhang, Huangqi Lan, Lingling Liang, Cenhan Huang, Tianzi Li
IF：
2.6070
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JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
PMID：
33750217
Mechanistic studies on protective effects of total flavonoids from Ilex latifolia Thunb. on UVB-radiated human keratinocyte cell line (HaCaT cells) based on network pharmacology and molecular docking technique
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Author：
Yunge Ma, Yingyan Li, Yike Yao, Tao Huang, Chong Lan, Liyan Li
IF：
2.6000
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PHOTOCHEMISTRY AND PHOTOBIOLOGY
PMID：
38644599
Tracheloside, the main constituent of the total lignan extract from Trachelospermi Caulis, inhibited rheumatoid arthritis via IL-17/MAPK signaling pathway
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Author：
He-Fei Wang, Zi-Hong Huang, Wei Zou, Chun-Hua Lai, Qin-Gang Tan
IF：
2.5000
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FITOTERAPIA
PMID：
39603466
Palmitic acid up regulates Gal‑3 and induces insulin resistance in macrophages by mediating the balance between KLF4 and NF‑κB
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Author：
Jing Li, Yu-Shan Mao, Fen Chen, Dong-Xia Xia, Tin-Qi Zhao
IF：
2.4470
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Experimental and Therapeutic Medicine
PMID：
34373714
Implications of the SNHG10/miR-665/RASSF5/NF-κB pathway in dihydromyricetin-mediated ischemic stroke protection
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Author：
Qi Zeng, Yan Xiao, Xueliang Zeng, Hai Xiao
IF：
2.3000
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PeerJ
PMID：
39726744
High level of C3 is associated with Th2 immune response and liver fibrosis in patients with schistosomiasis
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Author：
Xianmo Wang, Quan Gong, Hao Nie, Jiancheng Tu, Wen Fan, Xiaoping Tan
IF：
2.2000
Publish_to：
PARASITE IMMUNOLOGY
PMID：
38465509
Interference of periostin attenuates pathological changes,proinflammatory markers and renal fibrosis in diabetic kidney injury
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Author：
Duan Xiaoting, Chen Cheng, Liu Xiaoli, Wang Taoxia, Feng Shuning, Li Jianwei, Li Guiying
IF：
2.1640
Publish_to：
Genes & Genomics
PMID：
37248423
SOS1-IT1 silencing alleviates MPP+-induced neuronal cell injury through regulating the miR-124-3p/PTEN/AKT/mTOR pathway
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Author：
Jianhu Fan, Dahua Wu, Yuxing Guo, Zhongbao Yang
IF：
2.1160
Publish_to：
JOURNAL OF CLINICAL NEUROSCIENCE
PMID：
35279586
Circ_0059662 exerts a positive role in oxygen每glucose deprivation/reoxygenation-induced SK-N-SH cell injury
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Author：
An Yang, Xu Dan, Yuan Lei, Wen Ying
IF：
2.0000
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EXPERIMENTAL BRAIN RESEARCH
PMID：
37815551
Tripterygium wilfordii derivative LLDT-8 targets CD2 in the treatment of rheumatoid arthritis
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Author：
Yuan Li, Wufang Qi, Lei Yan, Mengmeng Wang, Linru Zhao
IF：
1.9000
Publish_to：
Biomedical Reports
PMID：
34429967
CircTLK1 Downregulation Attenuates High Glucose-Induced Human Mesangial Cell Injury by Blocking the AKT/NF-κB Pathway Through Sponging miR-126-5p/miR-204-5p
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Author：
Qiu Binghua, Qi Xin, Wang Juan
IF：
1.8900
Publish_to：
BIOCHEMICAL GENETICS
PMID：
34731387
Astragaloside IV relieves IL-1汕-induced human nucleus pulposus cells degeneration through modulating PI3K/Akt signaling pathway
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Author：
Lu Zhang, Junsheng Gao, Zhentao Li, Jun Liu, Chong Zhang, Jie Liu, Hui Dong, Wei Mei
IF：
1.6000
Publish_to：
MEDICINE
PMID：
37603510
Atorvastatin inhibits ischemia‒reperfusion-associated renal tubular cell ferroptosis by blocking the PGE2/EP4 signaling pathway
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Author：
Yang Jing, Zhou Rongrong, Zhou Mengjiao, Li Xinghuan
IF：
1.5000
Publish_to：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
PMID：
39920560
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