中文
英语
琥珀酸脱氢酶(SDH)活性检测试剂盒
检测方法:可见分光光度法 Spectrophotometer
英文名称:Succinate Dehydrogenase(SDH) Activity Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | 琥珀酸脱氢酶(SDH)活性检测试剂盒 |
| 英文名称 | Succinate Dehydrogenase(SDH) Activity Assay Kit |
| 检测方法 | 可见分光光度法 Spectrophotometer |
| 储存条件 | Store at -20℃，6 months |
| 规格 | 50T/48S |
| 单位 | 盒 |
测定意义：SDH（EC 1.3.5.1）广泛存在于动物、植物、微生物和培养细胞中。SDH 是线粒体的一种标志酶，位于线粒体内膜上的一种膜结合酶，是连接呼吸电子传递和氧化磷酸化的枢纽之一。此外，为多种原核细胞产能的呼吸链提供电子。
测定原理：SDH 催化琥珀酸脱氢生成延胡索酸，脱下的氢通过吩嗪二甲酯硫酸（PMS）传递还原2,6-二氯酚靛酚（DCPIP），并且在600nm 处具有特征吸收峰，通过600nm 吸光度的变化，测定2．6-DPIP 的还原速度，代表SDH 酶活性。
需自备的仪器和用品：可见分光光度计、水浴锅、台式离心机、可调式移液器、1 mL 玻璃比色皿、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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