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人白细胞介素6（高灵敏）检测试剂盒
英文名称:Human IL-6(High Sensitivity) ELISA Kit
应用: ELISA
反应性:Human
样品类型:血清/血浆/细胞培养上清
灵敏度:0.3 pg/ml
储存条件:2-8℃，6 months
产品简介
| 中文名称 | 人白细胞介素6（高灵敏）检测试剂盒 |
| 英文名称 | Human IL-6(High Sensitivity) ELISA Kit |
| 别名 | B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor; HSF; hybridoma growth factor; IFNB2; IFN-beta-2 |
| 储存条件 | 2-8℃，6 months |
| 检测目标 | IL-6 |
| 样品类型 | 血清/血浆/细胞培养上清 |
| 反应性 | Human |
| 反应类型 | Sandwich-ELISA |
| 定量/定性 | 定量检测 |
| 检测范围 | 0.625-40 pg/ml |
| 灵敏度 | 0.3 pg/ml |
| 特异性 | 不与人IL-6sR,人IL-9,人IL-10,小鼠IL-6,小鼠IL-10,小鼠IL-11,小鼠IL-12等反应。 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 反应时间 | 3.5h |
| Swiss Prot | P05231 |
| Gene ID | 3569 |
| 试剂盒类型 | 高灵敏ELISA试剂盒 |
| 检测原理 | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人IL-6单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人IL-6会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人IL-6抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人IL-6发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人IL-6，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人IL-6浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人IL-6的浓度。 |
| 规格 | 48T 96T |
| 应用 | ELISA |
| 疾病系列 | 肥胖研究相关因子 骨质疏松症研究相关因子 冠心病研究相关因子 慢阻肺COPD研究相关因子 骨关节炎相关因子 神经系统疾病相关因子 糖尿病研究相关因子 系统性红斑狼疮相关因子 哮喘相关因子 心血管疾病相关因子 |
| 研究领域 | 神经学 新陈代谢 肿瘤&癌症 免疫学 |
| 单位 | 盒 |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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2.6070
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JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
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33750217
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38644599
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2.5000
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FITOTERAPIA
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39603466
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2.3000
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PeerJ
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2.1160
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JOURNAL OF CLINICAL NEUROSCIENCE
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35279586
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Yuan Li, Wufang Qi, Lei Yan, Mengmeng Wang, Linru Zhao
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1.9000
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Biomedical Reports
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34429967
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Qiu Binghua, Qi Xin, Wang Juan
IF：
1.8900
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BIOCHEMICAL GENETICS
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34731387
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1.6000
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MEDICINE
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37603510
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