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琥珀酸脱氢酶(SDH)活性检测试剂盒
检测方法:微量法 Spectrophotometer/Microplate Reader
英文名称:Succinate Dehydrogenase(SDH) Activity Assay Kit
储存条件:Store at -20℃，6 months
产品简介
| 中文名称 | 琥珀酸脱氢酶(SDH)活性检测试剂盒 |
| 英文名称 | Succinate Dehydrogenase(SDH) Activity Assay Kit |
| 别名 | 琥珀酸脱氢酶试剂盒 SDH Kit 琥珀酸脱氢酶（SDH)试剂盒 琥珀酸脱氢酶（SDH)测试盒 |
| 检测方法 | 微量法 Spectrophotometer/Microplate Reader |
| 储存条件 | Store at -20℃，6 months |
| 规格 | 100T/96S |
| 单位 | 盒 |
测定意义：SDH（EC 1.3.5.1）广泛存在于动物、植物、微生物和培养细胞中。SDH是线粒体的一种标志酶，位于线粒体内膜上的一种膜结合酶，是连接呼吸电子传递和氧化磷酸化的枢纽之一。此外，为多种原核细胞产能的呼吸链提供电子。
测定原理：SDH催化琥珀酸脱氢生成延胡索酸，脱下的氢通过吩嗪二甲酯硫酸（PMS）传递还原2,6-二氯酚靛酚（DCPIP），并且在600nm处具有特征吸收峰，通过600nm吸光度的变化，测定2．6-DPIP的还原速度，代表SDH酶活性。
需要的仪器，耗材:可见分光光度计/酶标仪、水浴锅、台式离心机、可调式移液器、微量石英比色皿/96孔板、研钵、冰和蒸馏水。
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these
methods.
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乔磊
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4.1710
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32243488
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Zhi Ma, Shuo Qiu, Dong Zhang, Xinying Guo, Yuele Lu, Yongxian Fan, Xiaolong Chen
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4.1000
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PEST MANAGEMENT SCIENCE
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36658467
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Xiaoyu Liang, Lijun Zou, Wenxu Lian, Meng Wang, Ye Yang, Yu Zhang
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4.0100
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34879716
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Lili An, Nan Gong, Taoting Hu, Lan Wang, Mei Zhang, Minjia Huang, Gongzhen Chen, Ting Tang, Xin Liu
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3.9000
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Infection and Drug Resistance
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37546368
Novel heterocyclic amide derivatives containing a diphenylmethyl moiety: systematic optimizations, synthesis, antifungal activity and action mechanism
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Feng Peng, Jianqi Chai, Yue Xie, Lang Tai, Min Chen, Chunlong Yang
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3.8000
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PEST MANAGEMENT SCIENCE
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39344702
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3.5000
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Food Science & Nutrition
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39055233
The protective effect of baicalin on duck hepatitis A virus type 1-induced duck hepatic mitochondria dysfunction by activating nuclear erythroid 2-related factor 2/antioxidant responsive element signaling pathway
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Linglin Su, Rui Wang, Tianxin Qiu, Jinli Wang, Jinwu Meng, Jinyue Zhu, Deyun Wang, Yi Wu, Jiaguo Liu
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3.3520
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POULTRY SCIENCE
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33744612
Effects of exposure to nanoplastics on the gill of mussels Mytilus galloprovincialis: An integrated perspective from multiple biomarkers
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Xin Wang, Shengyuan Shao, Tianyu Zhang, Qianqian Zhang, Dinglong Yang, Jianmin Zhao
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3.3000
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MARINE ENVIRONMENTAL RESEARCH
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37708618
Subcellular-Level Mitochondrial Energy Metabolism Response in the Fat Body of the German Cockroach Fed Abamectin
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Lin-Yu Yang, Xiao-Jie Yang, Zi-Shun Zhao, Qi-Lin Zhang
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3.1390
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Insects
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36555001
Sub3 inhibits mycelia growth and aflatoxin production of Aspergillus flavus
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Author：
张威
IF：
3.1140
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Food Biophysics
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Effect of Red Visible Lighting on Postharvest Ripening of Bananas via the Regulation of Energy Metabolism
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Xinqun Zhou, Jianhu Cheng, Jing Sun, Shuzhen Guo, Xuexia Guo, Quan Chen, Xiaomei Wang, Xuan Zhu, Bangdi Liu
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3.1000
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Horticulturae
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The antibacterial mechanism of phenylacetic acid isolated from Bacillus megaterium L2 against Agrobacterium tumefaciens
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Hang Pan, Yang Xiao, Ailin Xie, Zhu Li, Haixia Ding, XiaoJu Yuan, Ran Sun, Qiuju Peng
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3.0610
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PeerJ
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36389424
Dimethyl itaconate inhibits LPS‑induced microglia inflammation and inflammasome‑mediated pyroptosis via inducing autophagy and regulating the Nrf‑2/HO‑1 signaling pathway
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Su Yang, Xingxing Zhang, Hengli Zhang, Xiangxiang Lin, Xijun Chen, Ying Zhang, Xiao Lin, Lijie Huang, Qichuan Zhuge
IF：
2.9520
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Molecular Medicine Reports
PMID：
34296312
Anti-fungal activity of a novel succinate dehydrogenase inhibitor pydiflumetofen against Bipolaris maydis
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Yang Sun, Kun Wang, Bingyun Yang, Junpeng Yang, Bing Liu, Xing Chen, Wende Liu, Yu Chen
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2.8000
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CROP PROTECTION
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Histopathological, hematological, and biochemical changes in high-latitude fish Phoxinus lagowskii exposed to hypoxia
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Yang Yuting, Wang Zhen, Wang Jing, Lyu Fengming, Xu Kexin, Mu Weijie
IF：
2.7940
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FISH PHYSIOLOGY AND BIOCHEMISTRY
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33860915
Atorvastatin reverses high cholesterol-induced cardiac remodelling and regulates mitochondrial quality-control in a cholesterol-independent manner: An experimental study
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Peng Zheng, Yanzi Ding, Feiyan Lu, Nannan Liu, Hengfang Wu, Zhiping Bian, Xiangjian Chen, Di Yang
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2.5570
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CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
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33891707
Icariin and its phosphorylated derivatives reduce duck hepatitis A virus serotype 1-induced oxidative stress and inflammatory damage in duck embryonic hepatocytes through mitochondrial regulation
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Wen Xiong, Rui Wang, Wensai Mao, Yi Wu, Deyun Wang, Yuanliang Hu, Jiaguo Liu
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2.5340
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RESEARCH IN VETERINARY SCIENCE
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34298287
Comparison of effects in sustained and diel-cycling hypoxia on hypoxia tolerance, histology, physiology and expression of clock genes in high latitude fish Phoxinus lagowskii
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Jing Wang, Yuting Yang, Zhen Wang, Kexin Xu, Xin Xiao, Weijie Mu
IF：
2.3200
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COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
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34166835
Baseline sensitivity and resistance analysis of fluopyram against Botrytis cinerea from tomato in Liaoning Province,China
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Liting Wu, Jiani Liu, Kai Wang, Shuang Pan, Zhiqiu Qi
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1.5000
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JOURNAL OF PHYTOPATHOLOGY
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bc0955，琥珀酸脱氢酶活性检测试剂盒，按照要求加入试剂一和试剂二后，离心后上清大概有多少?2023-08-03 17:22:01
您好，不同样本间会有差异，与样本破碎离心效果也有关，一般离心后有700-800微升的上清液，沉淀是未完全破碎的以及一些大的组织细胞碎片。